After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

Mouse TIGIT / VSTM3 Protein (His Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Mouse TIGIT/VSTM3 Protein Product Information
Synonym:ENSMUSG00000071552, Vstm3
Protein Construction:A DNA sequence encoding the extracellular domain of mouse TIGIT (NP_001139797.1) (Met 1-Gly 141) was expressed, with a C-terminal polyhistidine tag.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Mouse TIGIT/VSTM3 Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Bio-Activity:Immobilized mouse TIGIT-His at 10 μg/ml (100 μl/well) can bind mouse PVR-Fch (Cat:50259-M03H), The EC50 of mouse PVR-Fch (Cat:50259-M03H) is 0.31-0.73 μg/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Gly 26
Molecule Mass:The secreted recombinant mouse TIGIT comprises 127 amino acids and has a calculated molecular mass of 14.2 kDa. As a result of glycosylation, the apparent molecular mass of rmSIRPA is approximately 20-25 kDa in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Mouse TIGIT/VSTM3 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Mouse TIGIT / VSTM3 Protein (His Tag) SDS-PAGE
Other TIGIT/VSTM3 Recombinant Protein Products
TIGIT/VSTM3 Background

TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-10 and diminished the production of IL-12p40. In addition, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.

Product nameProduct name
Size / Price
Catalog: 50939-M08H-50
List Price:   (Save )
Price:      [How to order]
AvailabilityIn StockShipping instructions
All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.