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Mouse SELP / selectin P / P-selectin Protein (His Tag)

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Mouse SELP Protein Product Information
Synonym:SELP, Grmp
Protein Construction:A DNA sequence encoding the mouse SELP (Q01102) (Met1-Ala709) was expressed with a C-terminal polyhistidine tag.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Mouse SELP Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:Measured by the ability of the immobilized protein to support the adhesion of U937 cells.
When 5 x 10E4 cells/well are added to SELP coated plates (1 μg/mL and 100 μL/well), approximately >85% cells will adhere specifically after 60 minutes at 37℃.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Trp 42
Molecule Mass:The recombinant mouse SELP comprises 679 amino acids and has a predicted molecular mass of 74 kDa. The apparent molecular mass of the protein is approximately 116 kDa in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Mouse SELP Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Mouse SELP Protein SDS-PAGE
Mouse SELP / selectin P / P-selectin Protein (His Tag) SDS-PAGE
Mouse SELP / selectin P / P-selectin Protein (His Tag) SDS-PAGE
Other SELP Recombinant Protein Products
SELP / P Selectin Background

P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.

Mouse SELP / P Selectin References
  • Johnson-Tidey RR, et al. (1994) Increase in the adhesion molecule P-selectin in endothelium overlying atherosclerotic plaques. Coexpression with intercellular adhesion molecule-1. Am J Pathol. 144(5):952-61.
  • Walcheck B, et al. (1996) Neutrophil-neutrophil interactions under hydrodynamic shear stress involve L-selectin and PSGL-1. A mechanism that amplifies initial leukocyte accumulation of P-selectin in vitro. J Clin Invest. 98(5):1081-7.
  • Foreman KE, et al. (1994) C5a-induced expression of P-selectin in endothelial cells. J Clin Invest. 94(3):1147-55.
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    Catalog: 50737-M08H-50
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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.