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Mouse CD45 / PTPRC Protein (aa 453-1152, His & GST Tag)

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CD45/PTPRCProtein Product Information
Synonym:PTPRC, Ly-5
Protein Construction:A DNA sequence encoding the mouse PTPRC (Arg453-Ser1152) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Form & Shipping:Liquid. It is shipped out with blue ice.
CD45/PTPRCProtein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:1. The specific activity was determined to be 12306 nmol/min/mg using p-nitrophenyl phosphate as substrate (see protocol for kinase assay).
2. Measured by its ability to bind biotinylated Galectin-1 (Cat:10290-HNAE-E) in a functional ELISA.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant mouse PTPRC/GST chimera consists of 937 amino acids and has a calculated molecular mass of 108.2 kDa. The recombinant protein migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile 20mM Tris, 500mM NaCl, 0.5mM GSH, 3mM DTT, pH 7.4, 10% gly
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
CD45/PTPRCProtein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexs or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.

  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Irie-Sasaki J, et al. (2001) CD45 is a JAK phosphatase and negatively regulates cytokine receptor signaling. Nature. 409: 349-54.
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    • Mouse CD45 / PTPRC Protein (aa 453-1152, His & GST Tag) SDS-PAGE
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