After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

Mouse UCHL3 / UCH-L3 Protein (His Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Mouse UCHL3 Protein Product Information
Protein Construction:A DNA sequence encoding the mouse UCHL3 (Q9JKB1) (Glu 2-Ala 230) was expressed, with a polyhistide tag at the N-terminus.
Expressed Host:E. coli
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Mouse UCHL3 Protein QC Testing
Purity:> 97 % as determined by SDS-PAGE
Bio-Activity:Measured by the hydrolysis of UbiquitinAMC . The specific activity is >14000 pmoles/min/μg.
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant mouse UCHL3 consisting of 240 amino acids and has a calculated molecular mass of 27.5 kDa. rm UCHL1 migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 50mM Tris, 150mM NaCl, 20% glycerol, pH 7.7
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Mouse UCHL3 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Mouse UCHL3 Protein SDS-PAGE
Mouse UCHL3 / UCH-L3 Protein (His Tag) SDS-PAGE
Other UCHL3 Recombinant Protein Products
UCHL3 Background

Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.

Mouse UCHL3 References
  • Wood,M.A. et al., 2005, Hippocampus  15 (5):610-21.
  • Kwon,J. et al., 2006, Exp Anim  55 (1):35-43.
  • Setsuie,R. et al., 2009, Neurochem Int  54 (5-6):314-21.
  • Setsuie,R. et al., 2010, Neurochem Int  56 (8):911-8.
  • Product nameProduct name
    Size / Price
    Catalog: 50692-M07E-20
    List Price:   (Save )
    Price:      [How to order]
    AvailabilityIn StockShipping instructions
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.