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Mouse CES5 / Carboxylesterase-5 HEK293 Cell Lysate (WB positive control)

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Mouse CES5 Transfected / Overexpression Cell Lysate Product Information
Expressed Host:Human Cells
Product Description:Human Cell lysate that Mouse CES5 / Carboxylesterase-5 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
Sequence information:A DNA sequence encoding the extracellular domain of mouse CES5 (NP_766347.1) (Met 1-His 556) was expressed, with a C-terminal polyhistidine tag.
Predicted N Terminal:Gln 27
Molecule Mass:The secreted recombinant mouse CES5 comprises 541 amino acids and has a calculated molecular mass of 60.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 52 kDa band in SDS-PAGE under reducing conditions.
Mouse CES5 Transfected / Overexpression Cell Lysate Usage Guide
Preparation Method:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
Stability:Samples are stable for up to twelve months from date of receipt.
Recommend Usage:1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
Storage Buffer:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Storage Instruction:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
Application notes:Western blot (WB): Use at an assay dependent dilution.
Other Applications: Not tested.
Optimal dilutions/concentrations should be determined by the end user.
CES5/Carboxylesterase 5 Background

Carboxylesterase belongs to the class of serine hydrolases family which includes Chymotrypsin and Acetylcholinesterase. Carboxylesterase is involved in the chemical reaction, exerting its role in catalyzing the carboxylic ester and water to convert to an alcohol and a carboxylate. Carboxylesterase is a type of enzyme that capable of hydrolyzing a variety of carboxylic acid esters and it's widely distributed in cells especially in mammalian liver. Carboxylesterase 5 (CES5), also known as cauxin or CES7, is one of CES enzyme families exerting role in catalyzing hydrolytic and transesterfication reactions with broad substrat specifity. CES5 is reported in high concentrations in the urine of adult male cats, and within a protein complex of mammalian male epididymal fluids. Roles for carboxylesterase 5 may include regulating urinary levels of cat pheromone, catalyzing lipid transfer reactions within mammalian male reproductive fluids, and protecting neural tissue from drugs and xenobiotics.

Mouse CES5/Carboxylesterase 5 References
  • Holmes RS, et al. (2008) Mammalian carboxylesterase 5: comparative biochemistry and genomics. Comp Biochem Physiol Part D Genomics Proteomics. 3 (3): 196-204.
  • Tashiro F, et al. (2010) Maternal-effect gene Ces5 / Ooep / Moep19 / Floped is essential for oocyte cytoplasmic lattice formation and embryonic development at the maternal-zygotic stage transition. Genes to Cells. 15 (8): 813-28.
  • Mentlein R, et al. (1984) Comparative chemical and immunological characterization of five lipolytic enzymes (carboxylesterases) from rat liver microsomes. Arch Biochem Biophys. 234 (2): 612-21.
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    Catalog: 50620-M08HL-300
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