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Mouse Ephrin-B2 / EFNB2 Protein (Fc Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
EphrinB2/EFNB2Protein Product Information
Synonym:Efnb2, ELF-2, Epl5, Eplg5, Htk-L, LERK-5, Lerk5, NLERK-1
Protein Construction:A DNA sequence encoding the mouse EFNB2 (NP_034241.2) extracellular domain (Met 1-Ala 232) was expressed with the fused Fc region of human IgG1 at the C-terminus.
Species:Mouse
Expressed Host:Human Cells
Form & Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
EphrinB2/EFNB2Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity: Measured by its binding ability in a functional ELISA . Immobilized mouse EphB4 at 2 μg/ml (100 μl/well) can bind mouse EphrinB2 Fc with a linear range of 0.2-25 ng/ml .
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Arg 29
Molecule Mass:The recombinant mouse EFNB2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 445 amino acids and has a predicted molecular mass of 49 kDa. As a result of glycosylation, the apparent molecular mass of rmEFNB2/Fc monomer is approximately 55-60 kDa in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
EphrinB2/EFNB2Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
50598-M02H
Background

EphrinB2 also known as EFNB2 is a member of the ephrin family. EphrinB2 is involved in establishing arterial versus venous identity and perhaps in anastamosing arterial and venous vessels at their junctions. The transmembrane-associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB2 expression progressively extends from the arterial endothelium to surrounding smooth muscle cells and to pericytes, suggesting that ephrin-B2 may play an important role during formation of the arterial muscle wall.

References
  • Wang HU, et al. (1998) Molecular distinction and angiogenic interaction between embryonic arteries and veins revealed by ephrin-B2 and its receptor Eph-B4. Cell. 93(5): 741-53.
  • Gale NW, et al. (2001) Ephrin-B2 selectively marks arterial vessels and neovascularization sites in the adult, with expression in both endothelial and smooth-muscle cells. Dev Biol. 230(2): 151-60.
  • Shin D, et al. (2001) Expression of ephrinB2 identifies a stable genetic difference between arterial and venous vascular smooth muscle as well as endothelial cells, and marks subsets of microvessels at sites of adult neovascularization. Dev Biol. 230(2): 139-50.
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    Size / Price
    Catalog:50598-M02H-100
    List Price: $108.00  (Save $0.00)
    Price:$108.00      [How to order]
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    • Mouse Ephrin-B2 / EFNB2 Protein (Fc Tag) SDS-PAGE
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