|Datasheet||Specific References||Reviews||Related Products||Protocols|
|AW494418, EFL-2, Ehk1-L, Epl3, LERK-3, MGC129300, MGC129301|
|A DNA sequence encoding the mouse EFNA4 (NP_031936.2) without the pro peptide (Met 1-Gly 176) was fused with the Fc region of human IgG1 at the C-terminus.|
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|> 92 % as determined by SDS-PAGE|
|Measured by its binding ability in a functional ELISA.|
1. Immobilized human EphA7 at 2 μg/ml (100 μl/well) can bind mouse EphrinA4 Fc Chimera with a linear range of 0.08-10 ng/ml .
2. Immobilized mouse EphA7 at 2 μg/ml (100 μl/well) can bind mouse EphrinA4 Fc Chimera with a linear range of 0.08-10 ng/ml.
|< 1.0 EU per μg of the protein as determined by the LAL method|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|The recombinant mouse EFNA4/Fc is a disulfide-linked homodimer. The reduced monomer consists of 392 amino acids and has a predicted molecular mass of 43.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEFNA4/Fc monomer is approximately 52 kDa due to glycosylation.|
|Lyophilized from sterile PBS, pH 7.4|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
EPH-related receptor tyrosine kinase ligand 4 (Ephrin-A4) also known as EFNA4, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. Ephrin-A4/EFNA4 functions as a cell surface GPI-bound ligand for Eph receptor, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development.