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EpCAM / TROP-1 / TACSTD1 Antibody, Rabbit MAb

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EpCAMAntibody Product Information
Immunogen:Recombinant Mouse EpCAM / TROP-1 / TACSTD1 protein (Catalog#50591-M08H)
Clone ID:002
Ig Type:Rabbit IgG
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Mouse EpCAM / TROP-1 / TACSTD1 (rM EpCAM / TROP-1 / TACSTD1; Catalog#50591-M08H; NP_032558.2; Met 1-Thr 266).
EpCAMAntibody Usage Guide
Specificity:Mouse EpCAM / TROP-1 / TACSTD1
No cross-reactivity in ELISA with
Human EPCAM
Application:WB, ELISA, IHC-P

WB: 10-20 μg/mL

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse EPCAM. The detection limit for Mouse EPCAM is approximately 0.00245 ng/well.

IHC-P: 1-5 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Images
EpCAM / TROP-1 / TACSTD1 Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of mouse EpCAM in mouse intestine with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
EpCAM / TROP-1 / TACSTD1 Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of mouse EpCAM in mouse stomach with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
EpCAM / TROP-1 / TACSTD1 Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of mouse EpCAM in mouse uterus with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
Background

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â… transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.

References
  • Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
  • Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
  • Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
  • Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
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    Catalog:50591-R002-50
    List Price: $170.00  (Save $0.00)
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    Images
    • EpCAM / TROP-1 / TACSTD1 Antibody, Rabbit MAb, Immunohistochemistry
    • EpCAM / TROP-1 / TACSTD1 Antibody, Rabbit MAb, Immunohistochemistry
    • EpCAM / TROP-1 / TACSTD1 Antibody, Rabbit MAb, Immunohistochemistry
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