|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CystatinC / CST3 ORF mammalian expression plasmid, C-GFPSpark tag||HG10439-ACG|
|Human CystatinC / CST3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10439-ACR|
|Human CystatinC / CST3 ORF mammalian expression plasmid, C-Flag tag||HG10439-CF|
|Human CystatinC / CST3 ORF mammalian expression plasmid, C-His tag||HG10439-CH|
|Human CystatinC / CST3 ORF mammalian expression plasmid, C-Myc tag||HG10439-CM|
|Human CystatinC / CST3 ORF mammalian expression plasmid, C-HA tag||HG10439-CY|
|Human CystatinC / CST3 Gene cDNA clone plasmid||HG10439-M|
|Human CystatinC / CST3 ORF mammalian expression plasmid, N-Flag tag||HG10439-NF|
|Human CystatinC / CST3 ORF mammalian expression plasmid, N-His tag||HG10439-NH|
|Human CystatinC / CST3 ORF mammalian expression plasmid, N-Myc tag||HG10439-NM|
|Human CystatinC / CST3 ORF mammalian expression plasmid, N-HA tag||HG10439-NY|
|Human CystatinC / CST3 natural ORF mammalian expression plasmid||HG10439-UT|
|Learn more about expression Vectors|
Cystatin C, also known as Cystatin-3 (CST3) is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate. The mature, active form of human cystatin C is a single non-glycosylated polypeptide chain consisting of 120 amino acid residues, with a molecular mass of 13,343-13,359 Da, and containing four characteristic disulfide-paired cysteine residues. Cystatin C is a low-molecular-weight protein which has been proposed as a marker of renal function that could replace creatinine. Indeed, the concentration of Cystatin C is mainly determined by glomerular filtration and is particularly of interest in clinical settings where the relationship between creatinine production and muscle mass impairs the clinical performance of creatinine. Since the last decade, numerous studies have evaluated its potential use in measuring renal function in various populations. More recently, other potential developments for its clinical use have emerged. In almost all the clinical studies, Cystatin C demonstrated a better diagnostic accuracy than serum creatinine in discriminating normal from impaired kidney function, but controversial results have been obtained by comparing this protein with other indices of kidney disease, especially serum creatinine-based equations, such as early atherosclerosis, Alzheimer's dementia, vascular aneurysms, hyperhomocysteinaemia and other neurodegenerative diseases. Cystatin C could be a useful clinical tool to identify HIV-infected persons. In addition, its expression is up-regulated in malignance of certain tumor progression.