|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FCER1A ORF mammalian expression plasmid, C-GFPSpark tag||HG13193-ACG|
|Human FCER1A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13193-ACR|
|Human FCER1A ORF mammalian expression plasmid, C-Flag tag||HG13193-CF|
|Human FCER1A ORF mammalian expression plasmid, C-His tag||HG13193-CH|
|Human FCER1A ORF mammalian expression plasmid, C-Myc tag||HG13193-CM|
|Human FCER1A ORF mammalian expression plasmid, C-HA tag||HG13193-CY|
|Human FCER1A Gene cDNA clone plasmid||HG13193-G|
|Human FCER1A ORF mammalian expression plasmid, N-Flag tag||HG13193-NF|
|Human FCER1A ORF mammalian expression plasmid, N-His tag||HG13193-NH|
|Human FCER1A ORF mammalian expression plasmid, N-Myc tag||HG13193-NM|
|Human FCER1A ORF mammalian expression plasmid, N-HA tag||HG13193-NY|
|Human FCER1A natural ORF mammalian expression plasmid||HG13193-UT|
|Learn more about expression Vectors|
FcERI, also known as FCER1A, is the alpha subunit of the immunoglobulin epsilon receptor (IgE receptor). IgE receptor is a high affinity IgE receptor which plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. IgE receptor is comprised of an alpha subunit(FcERI), a beta subunit, and two gamma subunits. FcERI is glycosylated and contains 2 Ig-like (immunoglobulin-like) domains.