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Mouse IFNA2 / Interferon alpha 2 Protein (Fc Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
IFNA2Protein Product Information
Synonym:P23-139P14.4, Ifa2
Protein Construction:A DNA sequence encoding the mouse IFNA2 (P01573?) (Cys 24-Glu 190) was fused with the Fc region of human IgG1 at the N-terminus.
Species:Mouse
Expressed Host:Human Cells
Form & Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
IFNA2Protein QC Testing
Purity:> 88 % as determined by SDS-PAGE
Bio-Activity:1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 0.1-0.8 ng/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized mouse IFNAR1-His (Cat:50469-M08H) at 10 μg/ml (100 μl/well) can bind mouse Fc-IFNA2 (cat:50525-M01H), The EC50 of mouse Fc-IFNA2 (cat:50525-M01H) is 0.8-1.9 μg/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Glu
Molecule Mass:The recombinant mouse IFNA2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 427 amino acids and has a calculated molecular mass of 47.8 kDa. The apparent molecular mass of the monomer is approximately 52 kDa in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
IFNA2Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
50525-M01H
References
  • Wang, et al. (2004) Fever of recombinant human interferon-alpha is mediated by opioid domain interaction with opioid receptor inducing prostaglandin E2. J Neuroimmunol. 156(1-2): 107-12.
  • Groopman JE, et al. (1984) Recombinant alpha-2 interferon therapy for Kaposi's sarcoma associated with the acquired immunodeficiency syndrome. Ann Intern Med. 100(5): 671-6.
  • Krueger JM, et al. (1987) Interferon alpha-2 enhances slow-wave sleep in rabbits. Int J Immunopharmacol. 9(1): 23-30.
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    Size / Price
    Catalog:50525-M01H-20
    List Price: $58.00  (Save $0.00)
    Price:$58.00      [How to order]
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    • Mouse IFNA2 / Interferon alpha 2 Protein (Fc Tag) SDS-PAGE
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