|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CCDC134 ORF mammalian expression plasmid, C-GFPSpark tag||HG13168-ACG|
|Human CCDC134 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13168-ACR|
|Human CCDC134 ORF mammalian expression plasmid, C-Flag tag||HG13168-CF|
|Human CCDC134 ORF mammalian expression plasmid, C-His tag||HG13168-CH|
|Human CCDC134 ORF mammalian expression plasmid, C-Myc tag||HG13168-CM|
|Human CCDC134 ORF mammalian expression plasmid, C-HA tag||HG13168-CY|
|Human CCDC134 Gene cDNA clone plasmid||HG13168-G|
|Human CCDC134 ORF mammalian expression plasmid, N-Flag tag||HG13168-NF|
|Human CCDC134 ORF mammalian expression plasmid, N-His tag||HG13168-NH|
|Human CCDC134 ORF mammalian expression plasmid, N-Myc tag||HG13168-NM|
|Human CCDC134 ORF mammalian expression plasmid, N-HA tag||HG13168-NY|
|Human CCDC134 natural ORF mammalian expression plasmid||HG13168-UT|
|Learn more about expression Vectors|
Human coiled-coil domain containing 134 (CCDC134) is a 229 amino acids secretory protein. Coiled-coil domain is a motif in which alpha-helix are coiled together. It has been found in many types of proteins, including transcription factors, intermediate filaments and certain tRNA synthetases. Many proteins containing such motif CCDC134 are involved in important biological functions. CCDC134 is also considered as a novel human MAPK-regulating protein that can inhibit the MAPK pathway. This protein significantly inhibite Elk1 transcriptional activity. The coiled-coil domain is a ubiquitous protein motif that is often involved in oligomerization.