|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human MSLN ORF mammalian expression plasmid, C-GFPSpark tag||HG13128-ACG|
|Human MSLN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13128-ACR|
|Human MSLN ORF mammalian expression plasmid, C-Flag tag||HG13128-CF|
|Human MSLN ORF mammalian expression plasmid, C-His tag||HG13128-CH|
|Human MSLN ORF mammalian expression plasmid, C-Myc tag||HG13128-CM|
|Human MSLN ORF mammalian expression plasmid, C-HA tag||HG13128-CY|
|Human MSLN Gene cDNA clone plasmid||HG13128-G|
|Human MSLN natural ORF mammalian expression plasmid||HG13128-G-N|
|Human MSLN ORF mammalian expression plasmid, N-Flag tag||HG13128-NF|
|Human MSLN ORF mammalian expression plasmid, N-His tag||HG13128-NH|
|Human MSLN ORF mammalian expression plasmid, N-Myc tag||HG13128-NM|
|Human MSLN ORF mammalian expression plasmid, N-HA tag||HG13128-NY|
|Human MSLN natural ORF mammalian expression plasmid||HG13128-UT|
|Learn more about expression Vectors|
Megakaryocyte potentiating factor belongs to the mesothelin family. This family is comprised by several mammalian pre-pro-megakaryocyte potentiating factor precursor (MPF) or mesothelin proteins. Mesothelin is a glycosylphosphatidylinositol-linked glycoprotein highly expressed in mesothelial cells, mesotheliomas, and ovarian cancer, but the biological function of the protein is not known. Megakaryocyte potentiating factor is highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). It interacts with MUC16 and potentiates megakaryocyte colony formation in vitro. Megakaryocyte potentiating factor is secreted by several mesothelioma cell lines and is frequently elevated in the blood of patients with mesothelioma. Measurement of this protein may be useful in following the response of mesothelioma to treatment.