|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TNFSF4 ORF mammalian expression plasmid, C-GFPSpark tag||HG13127-ACG|
|Human TNFSF4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13127-ACR|
|Human TNFSF4 ORF mammalian expression plasmid, C-Flag tag||HG13127-CF|
|Human TNFSF4 ORF mammalian expression plasmid, C-His tag||HG13127-CH|
|Human TNFSF4 ORF mammalian expression plasmid, C-Myc tag||HG13127-CM|
|Human TNFSF4 ORF mammalian expression plasmid, C-HA tag||HG13127-CY|
|Human TNFSF4 Gene cDNA clone plasmid||HG13127-G|
|Human TNFSF4 ORF mammalian expression plasmid, N-Flag tag||HG13127-NF|
|Human TNFSF4 ORF mammalian expression plasmid, N-His tag||HG13127-NH|
|Human TNFSF4 ORF mammalian expression plasmid, N-Myc tag||HG13127-NM|
|Human TNFSF4 ORF mammalian expression plasmid, N-HA tag||HG13127-NY|
|Human TNFSF4 natural ORF mammalian expression plasmid||HG13127-UT|
|Learn more about expression Vectors|
OX-40L, also known as TNFSF4 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. OX-40L is an important costimulatory molecule that plays a crucial role in the regulation of T-cell-mediated immunity. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. OX-40L is a ligand for receptor TNFRSF4/OX4. It is found to play a role in T cell antigen-presenting cell (APC) interactions. In surface Ig- and CD40-stimulated B cells, this cytokine along with CD70 has been shown to provide CD28-independent costimulatory signals to T cells. This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells.