|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human KRT14 ORF mammalian expression plasmid, C-GFPSpark tag||HG13109-ACG|
|Human KRT14 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13109-ACR|
|Human KRT14 ORF mammalian expression plasmid, N-GFPSpark tag||HG13109-ANG|
|Human KRT14 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13109-ANR|
|Human KRT14 ORF mammalian expression plasmid, C-Flag tag||HG13109-CF|
|Human KRT14 ORF mammalian expression plasmid, C-His tag||HG13109-CH|
|Human KRT14 ORF mammalian expression plasmid, C-Myc tag||HG13109-CM|
|Human KRT14 ORF mammalian expression plasmid, C-HA tag||HG13109-CY|
|Human KRT14 Gene cDNA clone plasmid||HG13109-G|
|Human KRT14 ORF mammalian expression plasmid, N-Flag tag||HG13109-NF|
|Human KRT14 ORF mammalian expression plasmid, N-His tag||HG13109-NH|
|Human KRT14 ORF mammalian expression plasmid, N-Myc tag||HG13109-NM|
|Human KRT14 ORF mammalian expression plasmid, N-HA tag||HG13109-NY|
|Human KRT14 natural ORF mammalian expression plasmid||HG13109-UT|
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Cytokeratin 14, also known as Keratin 14 and K14, is a member of the keratin family. Cytokeratin 14 is a type I keratin. It is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Cytokeratin 14 is mainly expressed in the basal layer. It is also strongly expressed in the outer root sheath of anagen follicles. Cytokeratin 14 and keratin 5 may have a role in maintenance of cell proliferation potential in the basal layer of stratified epithelia, modulating phosphatidylinositol 3-kinase/Akt–mediated cell proliferation and/or Notch1-dependent cell differentiation. Cytokeratin 14 defect prevents it from working effectively with keratin 5 and interfering with the assembly of the keratin intermediate filament network. A disruption in this network makes keratinocytes fragile and prone to rupture. Minor trauma to the skin, such as rubbing or scratching, can cause these cells to break down, resulting in the formation of painful, fluid-filled blisters. Mutations in the K14 gene are also responsible for Naegeli-Franceschetti-Jadassohn syndrome and dermatopathia pigmentosa reticularis.