|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human RSPO2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13084-ACG|
|Human RSPO2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13084-ACR|
|Human RSPO2 ORF mammalian expression plasmid, C-Flag tag||HG13084-CF|
|Human RSPO2 ORF mammalian expression plasmid, C-His tag||HG13084-CH|
|Human RSPO2 ORF mammalian expression plasmid, C-Myc tag||HG13084-CM|
|Human RSPO2 ORF mammalian expression plasmid, C-HA tag||HG13084-CY|
|Human RSPO2 Gene cDNA clone plasmid||HG13084-G|
|Human RSPO2 ORF mammalian expression plasmid, N-Flag tag||HG13084-NF|
|Human RSPO2 ORF mammalian expression plasmid, N-His tag||HG13084-NH|
|Human RSPO2 ORF mammalian expression plasmid, N-Myc tag||HG13084-NM|
|Human RSPO2 ORF mammalian expression plasmid, N-HA tag||HG13084-NY|
|Human RSPO2 natural ORF mammalian expression plasmid||HG13084-UT|
|Learn more about expression Vectors|
R-spondin-2, also known as RSPO2, synergizes with Wnt to activate beta-catenin. RSPO2 is secreted proteins that regulate beta-catenin signaling. Activator of the beta-catenin signaling cascade leads to TCF-dependent gene activation. Action both in the canonical Wnt / beta- catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled / LRP receptors. Probably also acts as a ligand for frizzled and LRP receptors. The encoding gene Rspo2 was identified as a novel common integration site for the mouse mammary tumor virus in viral induced mouse mammary tumors. Rspo2 and Rspo2 / Wnt1 tumors contained many spindle cells, consistent with an epithelial-mesenchymal transformation phenotype. When Rspo2 and Rspo2 / Wnt1 tumor cells were transferred into naive mice, they exhibited greater metastatic activity than cells derived from Wnt1 tumors.