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Rabbit Anti-Mouse IgM / Immunoglobulin M Antibody, Rabbit PAb

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Mouse IgM Antibody Product Information
Immunogen:Recombinant Mouse IgM
Clone ID:
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified recombinant Mouse IgM protein. Total IgG was purified by Protein A affinity chromatography.
Mouse IgM Antibody Usage Guide
Specificity:Mouse IgM
Has cross-reactivity in ELISA with

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Mouse IgM. The detection limit for Mouse IgM is 0.00245 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other IgM Antibody Products
IgM Background

Antibodies, also known as Immunoglobulins ( Ig ), are gamma globulin proteins that are found in blood or other bodily fluids of vertebrates, and are used by the immune system to identify and neutralize foreign objects, such as bacteria and viruses. Antibodies can come in different varieties known as isotypes or classes. In placental mammals there are five antibody isotypes known as IgA, IgD, IgE, IgG and IgM. They are each named with an "" Ig "" prefix that stands for immunoglobulin, another name for antibody, and differ in their biological properties, functional locations and ability to deal with different antigens. IgM is expressed on the surface of B cells and in a secreted form with very high avidity. It eliminates pathogens in the early stages of B cell mediated immunity before there is sufficient IgG. IgM is by far the physically largest antibody in the human circulatory system. It is produced after an animal has been exposed to an antigen for an extended time or when an animal is exposed to an antigen for the second time. IgM forms polymers where multiple immunoglobulins are covalently linked together with disulfide bonds, mostly as a pentamer but also as a hexamer. Because each monomer has two antigen binding sites, a pentameric IgM has 10 binding sites. Typically, however, IgM cannot bind 10 antigens at the same time because the large size of most antigens hinders binding to nearby sites. Due to its polymeric nature, IgM possesses high avidity, and is particularly effective at complement activation.

Mouse IgM References
  • Litman, GW. et al., 1993, Mol. Biol. Evol. 10 (1): 60-72.
  • Eleonora, MF. et al., 2003, PLoS Biology. 1 (1): e16.
  • Woof, J. et al., 2004, Nat Rev Immunol. 4 (2): 89-99.
  • Pier, GB. et al., 2004, ASM Press. ISBN 1-55581-246-5.
  • Geisberger, R. et al., 2006, Immunology. 118 (4): 429-37.
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