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Mouse SRC Kinase / Proto-oncogene c-Src Protein (His & GST Tag)

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Mouse SRC Protein Product Information
Synonym:RP23-169M4.1, AW259666, pp60c-src
Protein Construction:A DNA sequence encoding the mouse SRC (NP_001020566.111) (Met 1-Leu 535) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Mouse SRC Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:1. The specific activity was determined to be 166 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate.
2. Measured by its binding ability in a functional ELISA. Immobilized recombinant Mouse SRC at 2 μg/ml (100 μl/well) can bind biotinylated human PTPRA (aa 174-793) with a linear range of 0.032-0.8 μg/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant mouse SRC/GST chimera consists of 773 amino acids and has a calculated molecular mass of 87.7 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Mouse SRC Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Mouse SRC Protein SDS-PAGE
Mouse SRC Kinase / Proto-oncogene c-Src Protein (His & GST Tag) SDS-PAGE
Mouse SRC Kinase / Proto-oncogene c-Src Protein (His & GST Tag) SDS-PAGE
Other SRC Recombinant Protein Products
SRC/Proto-oncogene c-Src Background

Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

Mouse SRC/Proto-oncogene c-Src References
  • Juan Ma. et al., 2003, Neuroscience Letters. 348 (3): 185-189.
  • Czernilofsky AP. et al., 1980, Nature. 287: 198-203.
  • Beischlag TV. et al., 2002, Molecular and cellular biology. 22 (12): 4319-33.
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    Catalog: 50311-M20B-20
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