|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SLPI ORF mammalian expression plasmid, C-GFPSpark tag||HG10343-ACG|
|Human SLPI ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10343-ACR|
|Human SLPI ORF mammalian expression plasmid, C-Flag tag||HG10343-CF|
|Human SLPI ORF mammalian expression plasmid, C-His tag||HG10343-CH|
|Human SLPI ORF mammalian expression plasmid, C-Myc tag||HG10343-CM|
|Human SLPI ORF mammalian expression plasmid, C-HA tag||HG10343-CY|
|Human SLPI Gene cDNA clone plasmid||HG10343-M|
|Human SLPI ORF mammalian expression plasmid, Flag tag||HG10343-M-F|
|Human SLPI natural ORF mammalian expression plasmid, HA tag||HG10343-M-Y|
|Human SLPI ORF mammalian expression plasmid, N-Flag tag||HG10343-NF|
|Human SLPI ORF mammalian expression plasmid, N-His tag||HG10343-NH|
|Human SLPI ORF mammalian expression plasmid, N-Myc tag||HG10343-NM|
|Human SLPI ORF mammalian expression plasmid, N-HA tag||HG10343-NY|
|Human SLPI natural ORF mammalian expression plasmid||HG10343-UT|
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Secretory leukoprotease inhibitor (SLPI), also called antileukoprotease (ALP), is a 12-kDa, nonglycosylated serine protease inhibitor present in mucous secretions. It is thought to play a role in protecting the mucosae from injury associated with inflammation. SLPI is locally produced by serous cells, including bronchial submucosal glands. Elafin and SLPI are members of larger families of proteins secreted predominantly at mucosal sites, and have been shown to be modulated in multiple pathological conditions. Elafin and SLPI are structurally related in that both have a fold with a four-disulfide core or whey acidic protein (WAP) domain responsible for inhibiting proteases. SLPI is a prominent innate immune protein of the respiratory tract, possessing serine protease inhibitor activity, antibacterial activity, and anti-inflammatory/immunomodulatory activity.