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Mouse REN1 / Renin-1 Protein (His Tag)

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Mouse RENIN Protein Product Information
Synonym:Ren1, D19352, Ren, Ren-1, Ren-A, Ren1c, Ren1d, Rn-1, Rnr
Protein Construction:A DNA sequence encoding the pro form of mouse REN1 (NP_112469.1) (Met 1-Arg 402) was fused with a polyhistidine tag at the C-terminus.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Mouse RENIN Protein QC Testing
Purity:> 97 % as determined by SDS-PAGE
Bio-Activity:1. Measured by its ability to cleave the fluorogenic peptide substrate 5­FAM/QXL™ 520 (Peti­Peterdi, J. et al., 2009, Physiology 24:88.). The specific activity is > 20 pmoles/min/μg.
2. Immobilized mouse REN1-His (Cat: 50279-M08H) at 10ug/ml (100 μl/well) can bind biotinylated human AGT-His (Cat: 10994-H08H) with a linear range of 31.25-250 ng/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Leu 22
Molecule Mass:The recombinant mouse REN1 consists of 392 amino acids and has a predicted molecular mass of 43.2 kDa. As a result of glycosylation, the apparent molecular mass of rmREN1 is approximately 48-55 kDa in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Mouse RENIN Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Mouse RENIN Protein SDS-PAGE
Mouse REN1 / Renin-1 Protein (His Tag) SDS-PAGE
Other RENIN Recombinant Protein Products
Renin Background

Mouse Renin-1, also known as Ren-1, Angiotensinogenase and Kidney renin, is a member of the peptidase A1 family. Renin-1 is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. Renin-1 is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It is expressed at relatively low levels in mouse SMG and kidney. Ren-2 is expressed at high levels in the mouse SMG and at very low levels, if at all, in the kidney. Ren-1 and Ren-2 are closely linked on mouse chromosome 1, show extensive homology in coding and noncoding regions and provide a model for studying the regulation of gene expression.

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Catalog: 50279-M08H-20
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