|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CD50 / ICAM3 ORF mammalian expression plasmid, C-GFPSpark tag||HG10333-ACG|
|Human CD50 / ICAM3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10333-ACR|
|Human CD50 / ICAM3 ORF mammalian expression plasmid, C-Flag tag||HG10333-CF|
|Human CD50 / ICAM3 ORF mammalian expression plasmid, C-His tag||HG10333-CH|
|Human CD50 / ICAM3 ORF mammalian expression plasmid, C-Myc tag||HG10333-CM|
|Human CD50 / ICAM3 ORF mammalian expression plasmid, C-HA tag||HG10333-CY|
|Human CD50 / ICAM3 Gene cDNA clone plasmid||HG10333-M|
|Human CD50 / ICAM3 ORF mammalian expression plasmid, N-Flag tag||HG10333-NF|
|Human CD50 / ICAM3 ORF mammalian expression plasmid, N-His tag||HG10333-NH|
|Human CD50 / ICAM3 ORF mammalian expression plasmid, N-Myc tag||HG10333-NM|
|Human CD50 / ICAM3 ORF mammalian expression plasmid, N-HA tag||HG10333-NY|
|Human CD50 / ICAM3 natural ORF mammalian expression plasmid||HG10333-UT|
|Learn more about expression Vectors|
The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes, and is thus an important molecule for the first immune response. ICAM-3 comprises of five immunoglobulin-like domains, and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. In addition, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase of cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker.