|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human HMGB1 / HMG1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10326-ACG|
|Human HMGB1 / HMG1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10326-ACR|
|Human HMGB1 / HMG1 ORF mammalian expression plasmid, N-GFPSpark tag||HG10326-ANG|
|Human HMGB1 / HMG1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10326-ANR|
|Human HMGB1 / HMG1 ORF mammalian expression plasmid, C-Flag tag||HG10326-CF|
|Human HMGB1 / HMG1 ORF mammalian expression plasmid, C-His tag||HG10326-CH|
|Human HMGB1 / HMG1 ORF mammalian expression plasmid, C-Myc tag||HG10326-CM|
|Human HMGB1 / HMG1 ORF mammalian expression plasmid, C-HA tag||HG10326-CY|
|Human HMGB1 / HMG1 Gene cDNA clone plasmid||HG10326-M|
|Human HMGB1 / HMG1 ORF mammalian expression plasmid, N-Flag tag||HG10326-NF|
|Human HMGB1 / HMG1 ORF mammalian expression plasmid, N-His tag||HG10326-NH|
|Human HMGB1 / HMG1 ORF mammalian expression plasmid, N-Myc tag||HG10326-NM|
|Human HMGB1 / HMG1 ORF mammalian expression plasmid, N-HA tag||HG10326-NY|
|Human HMGB1 / HMG1 natural ORF mammalian expression plasmid||HG10326-UT|
|Learn more about expression Vectors|
High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a DNA-binding nuclear protein, released actively following cytokine stimulation as well as passively during cell death. It is the prototypic damage-associated molecular pattern (DAMP) molecule and has been implicated in several inflammatory disorders. HMGB1 signals via the receptor for advanced glycation end-product (RAGE) and members of the toll-like receptor (TLR) family. The most prominent HMGB1 protein and mRNA expression arthritis is present in pannus regions, where synovial tissue invades articular cartilage and bone. HMGB1 promotes the activity of proteolytic enzymes, and osteoclasts need HMGB1 for functional maturation. As a non-histone nuclear protein, HMGB1 has a dual function. Inside the cell, HMGB1 binds DNA, regulating transcription and determining chromosomal architecture. Outside the cell, HMGB1 can serve as an alarmin to activate the innate system and mediate a wide range of physiological and pathological responses. Extracellular HMGB1 represents an optimal "necrotic marker" selected by the innate immune system to recognize tissue damage and initiate reparative responses. However, extracellular HMGB1 also acts as a potent pro-inflammatory cytokine that contributes to the pathogenesis of diverse inflammatory and infectious disorders. HMGB1 has been successfully therapeutically targeted in multiple preclinical models of infectious and sterile diseases including arthritis. As shown in studies on patients as well as animal models, HMGB1 can play an important role in the pathogenesis of rheumatic disease, including rheumatoid arthritis, systemic lupus erythematosus, and polymyositis among others. In addition, enhanced postmyocardial infarction remodeling in type 1 diabetes mellitus was partially mediated by HMGB1 activation.