|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human HB-EGF ORF mammalian expression plasmid, C-GFPSpark tag||HG10325-ACG|
|Human HB-EGF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10325-ACR|
|Human HB-EGF ORF mammalian expression plasmid, C-Flag tag||HG10325-CF|
|Human HB-EGF ORF mammalian expression plasmid, C-His tag||HG10325-CH|
|Human HB-EGF ORF mammalian expression plasmid, C-Myc tag||HG10325-CM|
|Human HB-EGF ORF mammalian expression plasmid, C-HA tag||HG10325-CY|
|Human HB-EGF Gene cDNA clone plasmid||HG10325-M|
|Human HB-EGF ORF mammalian expression plasmid, N-Flag tag||HG10325-NF|
|Human HB-EGF ORF mammalian expression plasmid, N-His tag||HG10325-NH|
|Human HB-EGF ORF mammalian expression plasmid, N-Myc tag||HG10325-NM|
|Human HB-EGF ORF mammalian expression plasmid, N-HA tag||HG10325-NY|
|Human HB-EGF natural ORF mammalian expression plasmid||HG10325-UT|
|Learn more about expression Vectors|
Heparin-binding EGF-like growth factor (HBEGF), a member of the EGF family of growth factors, exerts its biological activity through activation of the EGFR and other ErbB receptors. Soluble mature HBEGF is proteolytically processed from a larger membrane-anchored precursor and is a potent mitogen and chemotactic factor for fibroblasts, smooth muscle cells but not endothelial cells. HBEGF activates two EGF receptor subtypes, HER1 and HER4 and binds to cell surface HSPG. The transmembrane form of HBEGF is a juxtacrine growth and adhesion factor and is uniquely the receptor for diphtheria toxin. Both forms of HB-EGF participate in normal physiological processes and in pathological processes including tumor progression and metastasis, organ hyperplasia, and atherosclerotic disease. HBEGF participates in diverse biological processes, including heart development and maintenance, skin wound healing, eyelid formation, blastocyst implantation, progression of atherosclerosis and tumor formation, through the activation of signaling molecules downstream of ErbB receptors and interactions with molecules associated with HBEGF. tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta markedly increased HB-EGF mRNA levels in HUVEC by 12- and 7-fold, respectively, and induction of the gene by TNF-alpha was both dose- and time-dependent.