|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TXN2 ORF mammalian expression plasmid, C-GFPSpark tag||HG12557-ACG|
|Human TXN2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12557-ACR|
|Human TXN2 ORF mammalian expression plasmid, N-GFPSpark tag||HG12557-ANG|
|Human TXN2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12557-ANR|
|Human TXN2 ORF mammalian expression plasmid, C-Flag tag||HG12557-CF|
|Human TXN2 ORF mammalian expression plasmid, C-His tag||HG12557-CH|
|Human TXN2 ORF mammalian expression plasmid, C-Myc tag||HG12557-CM|
|Human TXN2 ORF mammalian expression plasmid, C-HA tag||HG12557-CY|
|Human TXN2 Gene cDNA clone plasmid||HG12557-G|
|Human TXN2 ORF mammalian expression plasmid, N-Flag tag||HG12557-NF|
|Human TXN2 ORF mammalian expression plasmid, N-His tag||HG12557-NH|
|Human TXN2 ORF mammalian expression plasmid, N-Myc tag||HG12557-NM|
|Human TXN2 ORF mammalian expression plasmid, N-HA tag||HG12557-NY|
|Human TXN2 natural ORF mammalian expression plasmid||HG12557-UT|
|Learn more about expression Vectors|
Thioredoxin-2, also known as TXN2, MTRX and TRX2, is a member of the thioredoxin family. Tryparedoxins (TXN) are thioredoxin-related proteins which, as trypanothione:peroxiredoxin oxidoreductases, constitute the trypanothione-dependent antioxidant defense and may also serve as substrates for ribonucleotide reductase in trypanosomatids. Thioredoxin-2 / TXN2 contains one thioredoxin domain. It is widely expressed in adult (at protein level) and fetal tissues. Human Thioredoxin-2 / TXN2 is a small redox protein important in cellular antioxidant defenses, as well as in the regulation of apoptosis. Thioredoxin-2 / TXN2 has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Thioredoxin-2 / TXN2 could be involved in the resistance to anti-tumor agents. It possesses a dithiol-reducing activity. Thioredoxin-2 / TXN2 plays an important role in protecting the mitochondria against oxidative stress and in sensitizing the cells to ROS-induced apoptosis. Mammalian Thioredoxin-2 / TXN2 is a mitochondrial isoform of highly evolutionary conserved thioredoxins. Thioredoxins are small ubiquitous protein-disulfide oxidoreductases implicated in a large variety of biological functions.