|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SerpinA3 ORF mammalian expression plasmid, C-GFPSpark tag||HG10307-ACG|
|Human SerpinA3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10307-ACR|
|Human SerpinA3 ORF mammalian expression plasmid, C-Flag tag||HG10307-CF|
|Human SerpinA3 ORF mammalian expression plasmid, C-His tag||HG10307-CH|
|Human SerpinA3 ORF mammalian expression plasmid, C-Myc tag||HG10307-CM|
|Human SerpinA3 ORF mammalian expression plasmid, C-HA tag||HG10307-CY|
|Human SerpinA3 Gene cDNA clone plasmid||HG10307-M|
|Human SerpinA3 ORF mammalian expression plasmid, N-Flag tag||HG10307-NF|
|Human SerpinA3 ORF mammalian expression plasmid, N-His tag||HG10307-NH|
|Human SerpinA3 ORF mammalian expression plasmid, N-Myc tag||HG10307-NM|
|Human SerpinA3 ORF mammalian expression plasmid, N-HA tag||HG10307-NY|
|Human SerpinA3 natural ORF mammalian expression plasmid||HG10307-UT|
|Learn more about expression Vectors|
Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a plasma alpha globulin glycoprotein, and is a member of serpin superfamily of the serine protease inhibitors consisting of at least 35 members. SerpinA3 has been demonstrated to inhibit the activity of certain serine proteases, such as cathepsin G found in neutrophils, and chymases present in mast cells, by inducing a major conformational rearrangement, and thus protects some tissues from damage caused by proteolytic enzymes. This enzyme is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease, and mutations of this gene have been observed in patients with Parkinson disease and chronic obstructive pulmonary disease. In addition, ACT gene polymorphism has been implicated with Alzheimer’s disease (AD), cerebral amyloid angiopathy (CAA), as well as stroke, since SerpinA3 is a major constituent of the plaques in AD and an inhibitor of amyloid beta peptide degradation.