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CD38 Antibody, Rabbit PAb, Antigen Affinity Purified

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CD38Antibody Product Information
Immunogen:Recombinant Mouse CD38 protein (Catalog#50191-M08H)
Clone ID:
Ig Type:Rabbit IgG
Concentration:1 μg/Test
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Mouse CD38 (rM CD38; Catalog#50191-M08H; NP_031672.2; Leu 45-Thr 304). CD38 specific IgG was purified by mouse CD38 affinity chromatography .
CD38Antibody Usage Guide
Specificity:Mouse CD38
Application:ELISA, IHC-P, FCM

ELISA: 0.5-1.0 μg/mL

This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Mouse CD38. The detection limit for Mouse CD38 is 0.00245 ng/well.

IHC-P: 0.1-2 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Images
CD38 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of mouse CD38 in mouse kidney with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to endotheliocyte.
CD38 Antibody, Rabbit PAb, Antigen Affinity Purified, Flow cytometric
[Click to enlarge image]
Caption:
Flow cytometric analysis of CD38 expression on spleen lymphocytes. BALB/c splenocytes were stained with Mouse CD38 Affinity Purified Polyclonal Ab (50191-RP02, 1 μg/test), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.
CD38 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of mouse CD38 in mouse heart with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to endotheliocyte.
Background

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 38 (CD38), also known as ADP-ribosyl cyclase, is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B and natural killer cells. It shares several characteristics with ADP-ribosyl cyclase 2 CD157. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. It also functions in cell adhesion, signal transduction and calcium signaling. CD38 has been used as a prognostic marker in leukemia. It can also be used to identify plasma cells.

References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
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    Catalog:50191-RP02-50
    List Price: $170.00  (Save $0.00)
    Price:$170.00      [How to order]
    AvailabilityIn Stock
    Images
    • CD38 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
    • CD38 Antibody, Rabbit PAb, Antigen Affinity Purified, Flow cytometric
    • CD38 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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