|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PDK1 ORF mammalian expression plasmid, C-GFPSpark tag||HG12312-ACG|
|Human PDK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12312-ACR|
|Human PDK1 ORF mammalian expression plasmid, N-GFPSpark tag||HG12312-ANG|
|Human PDK1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12312-ANR|
|Human PDK1 ORF mammalian expression plasmid, C-Flag tag||HG12312-CF|
|Human PDK1 ORF mammalian expression plasmid, C-His tag||HG12312-CH|
|Human PDK1 ORF mammalian expression plasmid, C-Myc tag||HG12312-CM|
|Human PDK1 ORF mammalian expression plasmid, C-HA tag||HG12312-CY|
|Human PDK1 Gene cDNA clone plasmid||HG12312-G|
|Human PDK1 ORF mammalian expression plasmid, N-Flag tag||HG12312-NF|
|Human PDK1 ORF mammalian expression plasmid, N-His tag||HG12312-NH|
|Human PDK1 ORF mammalian expression plasmid, N-Myc tag||HG12312-NM|
|Human PDK1 ORF mammalian expression plasmid, N-HA tag||HG12312-NY|
|Human PDK1 natural ORF mammalian expression plasmid||HG12312-UT|
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Pyruvate dehydrogenase kinase, isozyme 1, also known as [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial and PDK1, is a member of the PDK / BCKDK protein kinase family. PDK-1 is expressed predominantly in the heart. It contains one histidine kinase domain. Pyruvate dehydrogenase kinase (PDK) isoforms are molecular switches that downregulate the pyruvate dehydrogenase complex (PDC) by reversible phosphorylation in mitochondria. An inhibitory effect of lipoic acid on PDKs would result in less phosphorylation of E1 and hence increased PDC activity. At least two isoenzymic forms of pyruvate dehydrogenase kinase ( PDK-1 and PDK-2 ) may be involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase complex by phosphorylation. PDK-3 appears to have the highest specific activity among the three isoenzymes. PDK-1 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.