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Frizzled-1 / FZD1 Antibody, Rabbit PAb, Antigen Affinity Purified

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Mouse Frizzled-1/FZD1 Antibody Product Information
Immunogen:Recombinant Mouse FZD1 protein (Catalog#50092-M08H)
Clone ID:
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Mouse FZD1 / Frizzled-1 extracellular domain (rh FZD1; Catalog#50092-M08H; NP_067432.2; Met 1-His 248). FZD1 specific IgG was purified by mouse FZD1 affinity chromatography.
Mouse Frizzled-1/FZD1 Antibody Usage Guide
Specificity:Mouse FZD1 / Frizzled-1
Application:ELISA, IHC-P

ELISA: 0.5-1.0 μg/mL

This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Mouse FZD1. The detection limit for Mouse FZD1 is 0.00245 ng/well.

IHC-P: 0.1-1 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Mouse Frizzled-1/FZD1 Antibody IHC Application Image
[Click to enlarge image]
Immunochemical staining of mouse FZD1 in mouse intestine with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Frizzled-1 / FZD1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Other Frizzled-1/FZD1 Antibody Products
Frizzled-1/FZD1 Background

Frizzled-1, also known as FZD1, belongs to the G-protein coupled receptor Fz/Smo family. FZD1 contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. FZD1 is expressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney. Frizzled is a family of G protein-coupled receptor proteins that serve as receptors in the Wnt signaling pathway and other signaling pathways. When activated, Frizzled leads to activation of Dishevelled in the cytosol. Frizzled proteins and the genes encoding them have been identified in an array of animals, from sponges to humans. Frizzled proteins play key roles in governing cell polarity, embryonic development, formation of neural synapses, cell proliferation, and many other processes in developing and adult organisms. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes.