|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human MMP-12 ORF mammalian expression plasmid, C-GFPSpark tag||HG10266-ACG|
|Human MMP-12 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10266-ACR|
|Human MMP-12 ORF mammalian expression plasmid, C-Flag tag||HG10266-CF|
|Human MMP-12 ORF mammalian expression plasmid, C-His tag||HG10266-CH|
|Human MMP-12 ORF mammalian expression plasmid, C-Myc tag||HG10266-CM|
|Human MMP-12 ORF mammalian expression plasmid, C-HA tag||HG10266-CY|
|Human MMP-12 Gene cDNA clone plasmid||HG10266-M|
|Human MMP-12 ORF mammalian expression plasmid, N-Flag tag||HG10266-NF|
|Human MMP-12 ORF mammalian expression plasmid, N-His tag||HG10266-NH|
|Human MMP-12 ORF mammalian expression plasmid, N-Myc tag||HG10266-NM|
|Human MMP-12 ORF mammalian expression plasmid, N-HA tag||HG10266-NY|
|Human MMP-12 natural ORF mammalian expression plasmid||HG10266-UT|
|Learn more about expression Vectors|
Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes such as morphogenesis, differentiation, angiogenesis and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. Macrophage metalloelastase, also known as Matrix metalloproteinase-12, Macrophage elastase, MMP12, and MMP-12, is a secreted protein which belongs to the peptidase M10A family. MMP12 is a macrophage-secreted elastase that is highly induced in the liver and lung in response to S. mansoni eggs and contains four hemopexin-like domains. MMP12 is a proteolytic enzyme responsible for cleavage of plasminogen to angiotensin, which has an angiostatic effect. It may be involved in tissue injury and remodeling and has significant elastolytic activity. It may be related to prognosis in breast cancer patients. MMP12 promotes fibrosis by limiting the expression of specific ECM-degrading MMPs. Like MMP12, MMP13 expression is highly dependent on IL-13 and type I I-IL-4 receptor signaling. MMP12 is a potent proinflammatory and oncogenic molecule. MMP12 up-regulation plays a critical role in emphysema to lung cancer transition that is facilitated by inflammation.