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Mouse IL-34 Protein (His Tag)

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IL34Protein Product Information
Synonym:2010004A03Rik, AI593503
Protein Construction:A DNA sequence encoding the extracellular domain (Met 1-Pro 235) of mouse mIL34 (NP_001128572.1) precursor was expressed with a polyhistidine tag at the C-terminus.
Expressed Host:Human Cells
Form & Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
IL34Protein QC Testing
Purity:> 97 % as determined by SDS-PAGE
Bio-Activity:1. Measured by its ability to bind biotinylated recombinant mouse CSF1R in a functional ELISA.
2. Measured in a cell proliferation assay using M-NFS60 mouse myeloid cells. The ED50 for this effect is typically 10-50 ng/mL.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Asn 21
Molecule Mass:The secreted recombinant mouse mIL34 consists of 226 amino acids and has a predicted molecular mass of 26 kDa. Due to glycosylation, rm IL34 migrates as several bands with the MW of approximately 38 kDa or higher in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
IL34Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Interleukin 34 (IL-34), also known as uncharacterized protein C16 or f77 homolog, is a secreted cytokine that belongs to unknown cytokine family. IL-34 protein is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate and colon, and most abundant in the spleen. The colony-stimulating factor 1 receptor (CSF-1R) is identified as the receptor for IL-34. IL-34 increases growth or survival of immune cells known as monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.

  • Lin H, et al. (2008) Discovery of a cytokine and its receptor by functional screening of the extracellular proteome. Science. 320(5877): 807-11.
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    Price:$178.00      [How to order]
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    • Mouse IL-34 Protein (His Tag) SDS-PAGE
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