|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human METAP2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10245-ACG|
|Human METAP2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10245-ACR|
|Human METAP2 ORF mammalian expression plasmid, N-GFPSpark tag||HG10245-ANG|
|Human METAP2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10245-ANR|
|Human METAP2 ORF mammalian expression plasmid, C-Flag tag||HG10245-CF|
|Human METAP2 ORF mammalian expression plasmid, C-His tag||HG10245-CH|
|Human METAP2 ORF mammalian expression plasmid, C-Myc tag||HG10245-CM|
|Human METAP2 ORF mammalian expression plasmid, C-HA tag||HG10245-CY|
|Human METAP2 Gene cDNA clone plasmid||HG10245-M|
|Human METAP2 ORF mammalian expression plasmid, N-Flag tag||HG10245-NF|
|Human METAP2 ORF mammalian expression plasmid, N-His tag||HG10245-NH|
|Human METAP2 ORF mammalian expression plasmid, N-Myc tag||HG10245-NM|
|Human METAP2 ORF mammalian expression plasmid, N-HA tag||HG10245-NY|
|Human METAP2 natural ORF mammalian expression plasmid||HG10245-UT|
|Learn more about expression Vectors|
METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a a protein which belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.