|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human LDLR ORF mammalian expression plasmid, C-GFPSpark tag||HG10231-ACG|
|Human LDLR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10231-ACR|
|Human LDLR ORF mammalian expression plasmid, C-Flag tag||HG10231-CF|
|Human LDLR ORF mammalian expression plasmid, C-His tag||HG10231-CH|
|Human LDLR ORF mammalian expression plasmid, C-Myc tag||HG10231-CM|
|Human LDLR ORF mammalian expression plasmid, C-HA tag||HG10231-CY|
|Human LDLR Gene cDNA clone plasmid||HG10231-M|
|Human LDLR natural ORF mammalian expression plasmid||HG10231-M-N|
|Human LDLR ORF mammalian expression plasmid, N-Flag tag||HG10231-NF|
|Human LDLR ORF mammalian expression plasmid, N-His tag||HG10231-NH|
|Human LDLR ORF mammalian expression plasmid, N-Myc tag||HG10231-NM|
|Human LDLR ORF mammalian expression plasmid, N-HA tag||HG10231-NY|
|Human LDLR natural ORF mammalian expression plasmid||HG10231-UT|
|Learn more about expression Vectors|
LDL Receptor, also known as LDLR, is a mosaic protein which belongs to the Low density lipoprotein receptor gene family. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 840 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants.