|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FGF19 ORF mammalian expression plasmid, C-GFPSpark tag||HG12226-ACG|
|Human FGF19 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12226-ACR|
|Human FGF19 ORF mammalian expression plasmid, C-Flag tag||HG12226-CF|
|Human FGF19 ORF mammalian expression plasmid, C-His tag||HG12226-CH|
|Human FGF19 ORF mammalian expression plasmid, C-Myc tag||HG12226-CM|
|Human FGF19 ORF mammalian expression plasmid, C-HA tag||HG12226-CY|
|Human FGF19 Gene cDNA clone plasmid||HG12226-G|
|Human FGF19 ORF mammalian expression plasmid, N-Flag tag||HG12226-NF|
|Human FGF19 ORF mammalian expression plasmid, N-His tag||HG12226-NH|
|Human FGF19 ORF mammalian expression plasmid, N-Myc tag||HG12226-NM|
|Human FGF19 ORF mammalian expression plasmid, N-HA tag||HG12226-NY|
|Human FGF19 natural ORF mammalian expression plasmid||HG12226-UT|
|Learn more about expression Vectors|
FGF19, also known as FGF-19, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF19 interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by KL, KLB and heparan sulfate glycosaminoglycans that function as coreceptors. It interacts with KL and KLB directly. However, it interacts with FGFR4 in the presence of heparin, KL or KLB. FGF19 is involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. It also stimulates glucose uptake in adipocytes.