|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human KLK-13 ORF mammalian expression plasmid, C-GFPSpark tag||HG10199-ACG|
|Human KLK-13 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10199-ACR|
|Human KLK-13 ORF mammalian expression plasmid, C-Flag tag||HG10199-CF|
|Human KLK-13 ORF mammalian expression plasmid, C-His tag||HG10199-CH|
|Human KLK-13 ORF mammalian expression plasmid, C-Myc tag||HG10199-CM|
|Human KLK-13 ORF mammalian expression plasmid, C-HA tag||HG10199-CY|
|Human KLK-13 Gene cDNA clone plasmid||HG10199-M|
|Human KLK-13 ORF mammalian expression plasmid, Flag tag||HG10199-M-F|
|Human KLK-13 ORF mammalian expression plasmid, N-Flag tag||HG10199-NF|
|Human KLK-13 ORF mammalian expression plasmid, N-His tag||HG10199-NH|
|Human KLK-13 ORF mammalian expression plasmid, N-Myc tag||HG10199-NM|
|Human KLK-13 ORF mammalian expression plasmid, N-HA tag||HG10199-NY|
|Human KLK-13 natural ORF mammalian expression plasmid||HG10199-UT|
|Learn more about expression Vectors|
Human tissue kallikrein 13 (hK13), also known as KLK-L4 (kallikrein-like gene 4), is a member of the human tissue kallikrein family of serine proteases having diverse physiological functions in many tissues. The KLK13 gene resides on chromosome 19q13.3-4 along with other 14 members in a gene cluster and shares a high degree of homology. KLK13 is a trypsin-like, secreted serine protease expressed specifically in the testicular tissue including prostate, salivary gland, breast, and testis. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and may play a role in metastasis. KLK13 may be involved in the pathogenesis and/or progression of breast and ovary cancers, and is regarded as a novel cancer biomarker. In addition, KLK13 interacts and forms complexes with several serum protease inhibitors, such as alpha2-macroglobulin, and its expression is regulated by steroid hormones.