|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human S100B ORF mammalian expression plasmid, C-GFPSpark tag||HG10181-ACG|
|Human S100B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10181-ACR|
|Human S100B ORF mammalian expression plasmid, N-GFPSpark tag||HG10181-ANG|
|Human S100B ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10181-ANR|
|Human S100B ORF mammalian expression plasmid, C-Flag tag||HG10181-CF|
|Human S100B ORF mammalian expression plasmid, C-His tag||HG10181-CH|
|Human S100B ORF mammalian expression plasmid, C-Myc tag||HG10181-CM|
|Human S100B ORF mammalian expression plasmid, C-HA tag||HG10181-CY|
|Human S100B Gene cDNA clone plasmid||HG10181-M|
|Human S100B ORF mammalian expression plasmid, Flag tag||HG10181-M-F|
|Human S100B ORF mammalian expression plasmid, N-Flag tag||HG10181-NF|
|Human S100B ORF mammalian expression plasmid, N-His tag||HG10181-NH|
|Human S100B ORF mammalian expression plasmid, N-Myc tag||HG10181-NM|
|Human S100B ORF mammalian expression plasmid, N-HA tag||HG10181-NY|
|Human S100B natural ORF mammalian expression plasmid||HG10181-UT|
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S100B is a member of the S100 family of proteins containing two EF-hand-type calcium-binding motifs. S100B exerts both intracellular and extracellular functions. Intracellular S100B acts as a stimulator of cell proliferation and migration and an inhibitor of apoptosis and differentiation, which might have important implications during brain, cartilage and skeletal muscle development and repair, activation of astrocytes in the course of brain damage and neurodegenerative processes, and of cardiomyocyte remodeling after infarction, as well as in melanomagenesis and gliomagenesis. As an extracellular factor, S100B engages RAGE (receptor for advanced glycation end products) in a variety of cell types with different outcomes (i.e. beneficial or detrimental, pro-proliferative or pro-differentiative) depending on the concentration attained by the protein, the cell type and the microenvironment. This calcium binding astrocyte-specific cytokine, presents a marker of astrocytic activation and reflects CNS injury. The excellent sensitivity of S100B has enabled it to confirm the existence of subtle brain injury in patients with mild head trauma, strokes, and after successful resuscitation from cardiopulmonary arrest. Recent findings provide evidence, that S100B may decrease neuronal injury and/or contribute to repair following traumatic brain injury (TBI). Hence, S100B, far from being a negative determinant of outcome, as suggested previously in the human TBI and ischemia literature, is of potential therapeutic value that could improve outcome in patients who sustain various forms of acute brain damage.