|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IL1R8 ORF mammalian expression plasmid, C-GFPSpark tag||HG10177-ACG|
|Human IL1R8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10177-ACR|
|Human IL1R8 ORF mammalian expression plasmid, C-Flag tag||HG10177-CF|
|Human IL1R8 ORF mammalian expression plasmid, C-His tag||HG10177-CH|
|Human IL1R8 ORF mammalian expression plasmid, C-Myc tag||HG10177-CM|
|Human IL1R8 ORF mammalian expression plasmid, C-HA tag||HG10177-CY|
|Human IL1R8 Gene cDNA clone plasmid||HG10177-M|
|Human IL1R8 ORF mammalian expression plasmid, N-Flag tag||HG10177-NF|
|Human IL1R8 ORF mammalian expression plasmid, N-His tag||HG10177-NH|
|Human IL1R8 ORF mammalian expression plasmid, N-Myc tag||HG10177-NM|
|Human IL1R8 ORF mammalian expression plasmid, N-HA tag||HG10177-NY|
|Human IL1R8 natural ORF mammalian expression plasmid||HG10177-UT|
|Learn more about expression Vectors|
Interleukin-1 receptor accessory protein-like 1 (IL1RAPL1) is a member of interleukin-1 receptor family. The protein structurally comprises three extracellular immunoglobulin domains, which presumably mediate binding of an as yet unidentified ligand, a transmembrane region, and an intracellular domain, which is likely to enable signalling via the NFkB pathway. The means of signalling is almost certain to be identical to that used by the IL1R family and the more distally related Toll protein. L1RAPL1 protein physically interacts via its 150 aa C-terminal domain with neuronal calcium sensor-1 (NCS-1), a protein widely expressed in neurons and the related chromaffin and PC12 cells. IL1RAPL1 is an integral membrane protein responsible for a nonsyndromic form of mental retardation (MR). It is suggested to affect human cognitive ability to some extent, especially the memory and concentration capability.