|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PROS1 ORF mammalian expression plasmid, C-GFPSpark tag||HG12179-ACG|
|Human PROS1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12179-ACR|
|Human PROS1 ORF mammalian expression plasmid, C-Flag tag||HG12179-CF|
|Human PROS1 ORF mammalian expression plasmid, C-His tag||HG12179-CH|
|Human PROS1 ORF mammalian expression plasmid, C-Myc tag||HG12179-CM|
|Human PROS1 ORF mammalian expression plasmid, C-HA tag||HG12179-CY|
|Human PROS1 Gene cDNA clone plasmid||HG12179-G|
|Human PROS1 ORF mammalian expression plasmid, N-Flag tag||HG12179-NF|
|Human PROS1 ORF mammalian expression plasmid, N-His tag||HG12179-NH|
|Human PROS1 ORF mammalian expression plasmid, N-Myc tag||HG12179-NM|
|Human PROS1 ORF mammalian expression plasmid, N-HA tag||HG12179-NY|
|Human PROS1 natural ORF mammalian expression plasmid||HG12179-UT|
|Learn more about expression Vectors|
PROS1, also known as protein S, is a vitamin K-dependent plasma protein that functions as a cofactor for the anticoagulant protease, activated protein C (APC) to inhibit blood coagulation. PROS1 has two isoforms: a free, functionally active form and an inactive form complexed with C4b-binding protein. Besides its anticoagulant function, PROS1 also acts as an agonist for the tyrosine kinase receptors Tyro3, Axl, and Mer. The endothelium expresses Tyro3, Axl, and Mer and produces protein S. The interaction of protein S with endothelial cells and particularly its effects on angiogenesis have not yet been analyzed.