|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GFAP ORF mammalian expression plasmid, C-GFPSpark tag||HG12167-ACG|
|Human GFAP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12167-ACR|
|Human GFAP ORF mammalian expression plasmid, N-GFPSpark tag||HG12167-ANG|
|Human GFAP ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12167-ANR|
|Human GFAP ORF mammalian expression plasmid, C-Flag tag||HG12167-CF|
|Human GFAP ORF mammalian expression plasmid, C-His tag||HG12167-CH|
|Human GFAP ORF mammalian expression plasmid, C-Myc tag||HG12167-CM|
|Human GFAP ORF mammalian expression plasmid, C-HA tag||HG12167-CY|
|Human GFAP Gene cDNA clone plasmid||HG12167-G|
|Human GFAP ORF mammalian expression plasmid, N-Flag tag||HG12167-NF|
|Human GFAP ORF mammalian expression plasmid, N-His tag||HG12167-NH|
|Human GFAP ORF mammalian expression plasmid, N-Myc tag||HG12167-NM|
|Human GFAP ORF mammalian expression plasmid, N-HA tag||HG12167-NY|
|Human GFAP natural ORF mammalian expression plasmid||HG12167-UT|
|Learn more about expression Vectors|
GFAP is a cell-specific marker which belongs to the intermediate filament family. It can distinguish astrocytes from other glial cells during development. GFAP is expressed in cells lacking fibronectin. It is a type III intermediate filaments protein which contains three domains: the head, rod and tail domains. GFAP functions in many important entral nervous system (CNS) processes, including cell communication and the functioning of the blood brain barrier. Improper GFAP regulation can cause multiple disorders. Defects in GFAP is related to Alexander disease which is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes.