|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human C2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10154-ACG|
|Human C2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10154-ACR|
|Human C2 ORF mammalian expression plasmid, C-Flag tag||HG10154-CF|
|Human C2 ORF mammalian expression plasmid, C-His tag||HG10154-CH|
|Human C2 ORF mammalian expression plasmid, C-Myc tag||HG10154-CM|
|Human C2 ORF mammalian expression plasmid, C-HA tag||HG10154-CY|
|Human C2 Gene cDNA clone plasmid||HG10154-M|
|Human C2 ORF mammalian expression plasmid, Flag tag||HG10154-M-F|
|Human C2 ORF mammalian expression plasmid, N-Flag tag||HG10154-NF|
|Human C2 ORF mammalian expression plasmid, N-His tag||HG10154-NH|
|Human C2 ORF mammalian expression plasmid, N-Myc tag||HG10154-NM|
|Human C2 ORF mammalian expression plasmid, N-HA tag||HG10154-NY|
|Human C2 natural ORF mammalian expression plasmid||HG10154-UT|
|Learn more about expression Vectors|
Complement component C2 is part of the classical complement pathway which plays a major role in innate immunity against infection. C2 is a glycoprotein synthesized in liver hepatocytes and several other cell types in extrahepatic tissues. This pathway is triggered by a multimolecular complex C1, and subsequently the single-chain form of C2 is cleaved into two chains referred to C2a and C2b by activated C1. The second component of complement (C2) is a multi-domain serine protease that provides catalytic activity for the C3 and C5 convertases of the classical and lectin pathways of human complement. C4b and C2 was investigated by surface plasmon resonance. C2a containing a serine protease domain combines with complement component C4b to form the C3 convertase C4b2a which is responsible for C3 activation, and leads to the stimulation of adaptive immune responses via Lectin pathway. C2 bound to C4b is cleaved by classical (C1s) or lectin (MASP2) proteases to produce C4bC2a. C2 has the same serine protease domain as C4bC2a but in an inactive zymogen-like conformation, requiring cofactor-induced conformational change for activity. Deficiency of C2 (C2D) is the most common genetic deficiency of the complement system, and two types of C2D have been recognized in the context of specific MHC haplotypes. C2D in human is reported to increase susceptibility to infection, and is associated with certain autoimmune diseases, such as rheumatological disorders.