|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ACTA2 ORF mammalian expression plasmid, C-GFPSpark tag||HG12160-ACG|
|Human ACTA2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12160-ACR|
|Human ACTA2 ORF mammalian expression plasmid, N-GFPSpark tag||HG12160-ANG|
|Human ACTA2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12160-ANR|
|Human ACTA2 ORF mammalian expression plasmid, C-Flag tag||HG12160-CF|
|Human ACTA2 ORF mammalian expression plasmid, C-His tag||HG12160-CH|
|Human ACTA2 ORF mammalian expression plasmid, C-Myc tag||HG12160-CM|
|Human ACTA2 ORF mammalian expression plasmid, C-HA tag||HG12160-CY|
|Human ACTA2 Gene cDNA clone plasmid||HG12160-G|
|Human ACTA2 natural ORF mammalian expression plasmid, HA tag||HG12160-G-Y|
|Human ACTA2 ORF mammalian expression plasmid, N-Flag tag||HG12160-NF|
|Human ACTA2 ORF mammalian expression plasmid, N-His tag||HG12160-NH|
|Human ACTA2 ORF mammalian expression plasmid, N-Myc tag||HG12160-NM|
|Human ACTA2 ORF mammalian expression plasmid, N-HA tag||HG12160-NY|
|Human ACTA2 natural ORF mammalian expression plasmid||HG12160-UT|
|Learn more about expression Vectors|
Actins are globular multi-functional proteins which can be detected in all eukaryotic cells. In vertebrates, there are three main groups of actins that possess slightly different functions: alpha, beta, and gamma. The alpha actins, found in muscle tissues, are a major constituent of the contractile apparatus. Beta-actin, found at the expanding edge of cells, uses the projection of its cellular structure as its mean of mobility. Gamma-actin is found in the filaments of stress fibres. ACTA2 is an alpha actin that is found in skeletal muscle. Expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.