|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ULBP2 ORF mammalian expression plasmid, C-GFPSpark tag||HG12143-ACG|
|Human ULBP2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12143-ACR|
|Human ULBP2 ORF mammalian expression plasmid, C-Flag tag||HG12143-CF|
|Human ULBP2 ORF mammalian expression plasmid, C-His tag||HG12143-CH|
|Human ULBP2 ORF mammalian expression plasmid, C-Myc tag||HG12143-CM|
|Human ULBP2 ORF mammalian expression plasmid, C-HA tag||HG12143-CY|
|Human ULBP2 Gene cDNA clone plasmid||HG12143-G|
|Human ULBP2 ORF mammalian expression plasmid, N-Flag tag||HG12143-NF|
|Human ULBP2 ORF mammalian expression plasmid, N-His tag||HG12143-NH|
|Human ULBP2 ORF mammalian expression plasmid, N-Myc tag||HG12143-NM|
|Human ULBP2 ORF mammalian expression plasmid, N-HA tag||HG12143-NY|
|Human ULBP2 natural ORF mammalian expression plasmid||HG12143-UT|
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NKG2D ligand 2, also known as N2DL-2, NKG2DL2, ALCAN-alpha, Retinoic acid early transcript 1H, UL16-binding protein 2, ULBP2 and N2DL2, is cell membrane protein which belongs to the MHC class I family. ULBP2 / N2DL-2 is expressed in various types of cancer cell lines and in the fetus, but not in normal tissues. ULBP2 / N2DL-2 is a ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway.