|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FABP2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10130-ACG|
|Human FABP2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10130-ACR|
|Human FABP2 ORF mammalian expression plasmid, N-GFPSpark tag||HG10130-ANG|
|Human FABP2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10130-ANR|
|Human FABP2 ORF mammalian expression plasmid, C-Flag tag||HG10130-CF|
|Human FABP2 ORF mammalian expression plasmid, C-His tag||HG10130-CH|
|Human FABP2 ORF mammalian expression plasmid, C-Myc tag||HG10130-CM|
|Human FABP2 ORF mammalian expression plasmid, C-HA tag||HG10130-CY|
|Human FABP2 Gene cDNA clone plasmid||HG10130-M|
|Human FABP2 ORF mammalian expression plasmid, N-Flag tag||HG10130-NF|
|Human FABP2 ORF mammalian expression plasmid, N-His tag||HG10130-NH|
|Human FABP2 ORF mammalian expression plasmid, N-Myc tag||HG10130-NM|
|Human FABP2 ORF mammalian expression plasmid, N-HA tag||HG10130-NY|
|Human FABP2 natural ORF mammalian expression plasmid||HG10130-UT|
|Learn more about expression Vectors|
Fatty acid binding protein (FABP) is one of the intracellular proteins, with a low molecular weight of approximately 15 kDa, that plays important roles in the transportation and metabolism of long-chain fatty acids. FABP family proteins could be used as tissue specific injury marker based on the following characteristics of FABP. The intestinal fatty acid binding protein (I-FABP), or fatty acid-binding protein 2 (FABP2), an intracellular protein expressed only in the intestine, involved in the absorption and intracellular transport of dietary long chain fatty acids. The FABP2 gene is proposed as a candidate gene for diabetes because the protein it codes is involved in fatty acid (FA) absorption and metabolism. Numerous studies have assessed FABP2 gene variants. A transition of G to A at codon 54 of FABP2 results in an amino acid substitution (Ala54 to Thr54), which is common in diverse populations and results in increased FA absorption in vivo. Some evidence indicates that this variant may be associated with type 2 diabetes. This polymorphism was associated with some cardiovascular risk factors. The cytosolic human intestinal fatty acid binding protein (hFABP2) is proposed to be involved in intestinal absorption of long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.