|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human VHR ORF mammalian expression plasmid, C-GFPSpark tag||HG10114-ACG|
|Human VHR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10114-ACR|
|Human VHR ORF mammalian expression plasmid, N-GFPSpark tag||HG10114-ANG|
|Human VHR ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10114-ANR|
|Human VHR ORF mammalian expression plasmid, C-Flag tag||HG10114-CF|
|Human VHR ORF mammalian expression plasmid, C-His tag||HG10114-CH|
|Human VHR ORF mammalian expression plasmid, C-Myc tag||HG10114-CM|
|Human VHR ORF mammalian expression plasmid, C-HA tag||HG10114-CY|
|Human VHR Gene cDNA clone plasmid||HG10114-M|
|Human VHR ORF mammalian expression plasmid, N-Flag tag||HG10114-NF|
|Human VHR ORF mammalian expression plasmid, N-His tag||HG10114-NH|
|Human VHR ORF mammalian expression plasmid, N-Myc tag||HG10114-NM|
|Human VHR ORF mammalian expression plasmid, N-HA tag||HG10114-NY|
|Human VHR natural ORF mammalian expression plasmid||HG10114-UT|
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Vaccinia H1-related phosphatase (VHR) is classified as a dual-specificity phosphatase (DUSP), and the other name is dual-specificity phosphatase 3 (DUSP3). DUSPs are a heterogeneous group of protein phosphatases that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. Unlike typical DUSPs, VHR lacks mitogen-activated protein kinase (MAPK)-binding domain, and shows poor activity against MAPKs. VHR often act on bisphosphorylated protein substrates, it displays a strong preference for dephosphorylating phosphotyrosine residues over phosphothreonine residues. VHR has been identified as a novel regulator of extracellular regulated kinases (ERKs). VHR is responsible for the rapid inactivation of ERK following stimulation and for its repression in quiescent cells. VHR is a negative regulator of the Erk and Jnk pathways in T cells and, therefore, may play a role in aspects of T lymphocyte physiology that depend on these kinases.