|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PRSS27 ORF mammalian expression plasmid, C-GFPSpark tag||HG12116-ACG|
|Human PRSS27 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12116-ACR|
|Human PRSS27 ORF mammalian expression plasmid, C-Flag tag||HG12116-CF|
|Human PRSS27 ORF mammalian expression plasmid, C-His tag||HG12116-CH|
|Human PRSS27 ORF mammalian expression plasmid, C-Myc tag||HG12116-CM|
|Human PRSS27 ORF mammalian expression plasmid, C-HA tag||HG12116-CY|
|Human PRSS27 Gene cDNA clone plasmid||HG12116-G|
|Human PRSS27 ORF mammalian expression plasmid, N-Flag tag||HG12116-NF|
|Human PRSS27 ORF mammalian expression plasmid, N-His tag||HG12116-NH|
|Human PRSS27 ORF mammalian expression plasmid, N-Myc tag||HG12116-NM|
|Human PRSS27 ORF mammalian expression plasmid, N-HA tag||HG12116-NY|
|Human PRSS27 natural ORF mammalian expression plasmid||HG12116-UT|
|Learn more about expression Vectors|
The name "Pancreasin" because it is transcribed strongly in the pancreas. This secreted, tryptic serine protease, also known as Marapsin or PRSS27 (Protease, serine, 27), which is a member of the peptidase S1 family. Pancreasin is inhibited by benzamidine and leupeptin but resists several classic inhibitors of trypsin. Marapsin was constitutively expressed in nonkeratinizing stratified squamous epithelia of human esophagus, tonsil, cervix, larynx, and cornea. In fact, marapsin was the second most strongly up-regulated protease in psoriatic lesions, where expression was localized to the upper region of the hyperplastic epidermis. Similarly, in the hyperproliferative epithelium of regenerating murine skin wounds, marapsin localized to the suprabasal layers, where keratinocytes undergo squamous differentiation. Marapsin's restricted expression, localization, and cytokine-inducible expression suggest a role in the terminal differentiation of keratinocytes in hyperproliferating squamous epithelia.