|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TNFRSF25 ORF mammalian expression plasmid, C-GFPSpark tag||HG12095-ACG|
|Human TNFRSF25 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12095-ACR|
|Human TNFRSF25 ORF mammalian expression plasmid, C-Flag tag||HG12095-CF|
|Human TNFRSF25 ORF mammalian expression plasmid, C-His tag||HG12095-CH|
|Human TNFRSF25 ORF mammalian expression plasmid, C-Myc tag||HG12095-CM|
|Human TNFRSF25 ORF mammalian expression plasmid, C-HA tag||HG12095-CY|
|Human TNFRSF25 Gene cDNA clone plasmid||HG12095-G|
|Human TNFRSF25 ORF mammalian expression plasmid, N-Flag tag||HG12095-NF|
|Human TNFRSF25 ORF mammalian expression plasmid, N-His tag||HG12095-NH|
|Human TNFRSF25 ORF mammalian expression plasmid, N-Myc tag||HG12095-NM|
|Human TNFRSF25 ORF mammalian expression plasmid, N-HA tag||HG12095-NY|
|Human TNFRSF25 natural ORF mammalian expression plasmid||HG12095-UT|
|Learn more about expression Vectors|
Tumor necrosis factor receptor superfamily, member 25 (TNFRSF25), also known as Death receptor 3 (DR3) or TNFRSF12 is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. TNFRSF25/DR3/TNFRSF12 has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this TNFRSF25 encoding gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.