|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CD166 / ALCAM ORF mammalian expression plasmid, C-GFPSpark tag||HG10045-ACG|
|Human CD166 / ALCAM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10045-ACR|
|Human CD166 / ALCAM ORF mammalian expression plasmid, C-Flag tag||HG10045-CF|
|Human CD166 / ALCAM ORF mammalian expression plasmid, C-His tag||HG10045-CH|
|Human CD166 / ALCAM ORF mammalian expression plasmid, C-Myc tag||HG10045-CM|
|Human CD166 / ALCAM ORF mammalian expression plasmid, C-HA tag||HG10045-CY|
|Human CD166 / ALCAM Gene cDNA clone plasmid||HG10045-M|
|Human CD166 / ALCAM ORF mammalian expression plasmid, N-Flag tag||HG10045-NF|
|Human CD166 / ALCAM ORF mammalian expression plasmid, N-His tag||HG10045-NH|
|Human CD166 / ALCAM ORF mammalian expression plasmid, N-Myc tag||HG10045-NM|
|Human CD166 / ALCAM ORF mammalian expression plasmid, N-HA tag||HG10045-NY|
|Human CD166 / ALCAM natural ORF mammalian expression plasmid||HG10045-UT|
|Learn more about expression Vectors|
Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.