|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PTGS2 ORF mammalian expression plasmid, C-GFPSpark tag||HG12036-ACG|
|Human PTGS2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12036-ACR|
|Human PTGS2 ORF mammalian expression plasmid, N-GFPSpark tag||HG12036-ANG|
|Human PTGS2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12036-ANR|
|Human PTGS2 ORF mammalian expression plasmid, C-Flag tag||HG12036-CF|
|Human PTGS2 ORF mammalian expression plasmid, C-His tag||HG12036-CH|
|Human PTGS2 ORF mammalian expression plasmid, C-Myc tag||HG12036-CM|
|Human PTGS2 ORF mammalian expression plasmid, C-HA tag||HG12036-CY|
|Human PTGS2 Gene cDNA clone plasmid||HG12036-G|
|Human PTGS2 ORF mammalian expression plasmid, N-Flag tag||HG12036-NF|
|Human PTGS2 ORF mammalian expression plasmid, N-His tag||HG12036-NH|
|Human PTGS2 ORF mammalian expression plasmid, N-Myc tag||HG12036-NM|
|Human PTGS2 ORF mammalian expression plasmid, N-HA tag||HG12036-NY|
|Human PTGS2 natural ORF mammalian expression plasmid||HG12036-UT|
|Learn more about expression Vectors|
PTGS2, also known as COX-2, is s component of Prostaglandin-endoperoxide synthase (PTGS). PTGS, also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. PTGS2 is over expressed in many cancers. The overexpression of PTGS2 along with increased angiogenesis and GLUT-1 expression is significantly associated with gallbladder carcinomas. Furthermore the product of COX-2, PGH2 is converted by prostaglandin E2 synthase into PGE2, which in turn can stimulate cancer progression. Consequently inhibiting COX-2 may have benefit in the prevention and treatment of these types of cancer. PTGS2 is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. It mediates the formation of prostaglandins from arachidonate and may have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.