|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human 4EBP1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10022-ACG|
|Human 4EBP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10022-ACR|
|Human 4EBP1 ORF mammalian expression plasmid, N-GFPSpark tag||HG10022-ANG|
|Human 4EBP1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10022-ANR|
|Human 4EBP1 ORF mammalian expression plasmid, C-Flag tag||HG10022-CF|
|Human 4EBP1 ORF mammalian expression plasmid, C-His tag||HG10022-CH|
|Human 4EBP1 ORF mammalian expression plasmid, C-Myc tag||HG10022-CM|
|Human 4EBP1 ORF mammalian expression plasmid, C-HA tag||HG10022-CY|
|Human 4EBP1 Gene cDNA clone plasmid||HG10022-M|
|Human 4EBP1 ORF mammalian expression plasmid, N-Flag tag||HG10022-NF|
|Human 4EBP1 ORF mammalian expression plasmid, N-His tag||HG10022-NH|
|Human 4EBP1 ORF mammalian expression plasmid, N-Myc tag||HG10022-NM|
|Human 4EBP1 ORF mammalian expression plasmid, N-HA tag||HG10022-NY|
|Human 4EBP1 natural ORF mammalian expression plasmid||HG10022-UT|
|Learn more about expression Vectors|
The translational suppressor eIF4E binding protein-1, 4E-BP1 functions as a key regulator in cellular growth, differentiation, apoptosis and survival. The Eif4ebp1 gene, encoding 4E-BP1, is a direct target of a transcription factor activating transcription factor-4 (ATF4), a master regulator of gene expression in stress responses. 4E-BP1 is characterized by its capacity to bind specifically to eIF4E and inhibit its interaction with eIF4G. Phosphorylation of 4E-BP1 regulates eIF4E availability, and therefore, cap-dependent translation, in cell stress. Binding of eIF4E to eIF4G is inhibited in a competitive manner by 4E-BP1. Phosphorylation of 4E-BP1 decreases the affinity of this protein for eIF4E, thus favouring the binding of eIF4G and enhancing translation. 4E-BP1 is important for beta-cell survival under endoplasmic reticulum (ER) stress. 4E-BP1 mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. Recently, 4E-BP1 was found to be a key factor, which converges several oncogenic signals, phosphorylates the molecules, and drives the downstream proliferative signals. Recent studies showed that high expression of phosphorylated 4E-BP-1 (p-4E-BP1) is associated with poor prognosis, tumor progression, or nodal metastasis in different human cancers.