|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CD309 / VEGFR2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10012-ACG|
|Human CD309 / VEGFR2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10012-ACR|
|Human CD309 / VEGFR2 ORF mammalian expression plasmid, C-Flag tag||HG10012-CF|
|Human CD309 / VEGFR2 ORF mammalian expression plasmid, C-His tag||HG10012-CH|
|Human CD309 / VEGFR2 ORF mammalian expression plasmid, C-Myc tag||HG10012-CM|
|Human CD309 / VEGFR2 ORF mammalian expression plasmid, C-HA tag||HG10012-CY|
|Human CD309 / VEGFR2 ORF mammalian expression plasmid, N-Flag tag||HG10012-NF|
|Human CD309 / VEGFR2 ORF mammalian expression plasmid, N-His tag||HG10012-NH|
|Human CD309 / VEGFR2 ORF mammalian expression plasmid, N-Myc tag||HG10012-NM|
|Human CD309 / VEGFR2 ORF mammalian expression plasmid, N-HA tag||HG10012-NY|
|Human CD309 / VEGFR2 natural ORF mammalian expression plasmid||HG10012-UT|
|Learn more about expression Vectors|
VEGFR2, also called as KDR or Flk-1, is identified as the receptor for VEGF and VEGFC and an early marker for endothelial cell progenitors, whose expression is restricted to endothelial cells in vivo. VEGFR2 was shown to be the primary signal transducer for angiogenesis and the development of pathological conditions such as cancer and diabetic retinopathy. It has been shown that VEGFR2 is expressed mainly in the endothelial cells, and the expression is upregulated in the tumor vasculature. Thus the inhibition of VEGFR2 activity and its downstream signaling are important targets for the treatment of diseases involving angiogenesis. VEGFR2 transduces the major signals for angiogenesis via its strong tyrosine kinase activity. However, unlike other representative tyrosine kinase receptors, VEGFR2 does not use the Ras pathway as a major downstream signaling but rather uses the phospholipase C-protein kinase C pathway to signal mitogen-activated protein (MAP)-kinase activation and DNA synthesis. VEGFR2 is a direct and major signal transducer for pathological angiogenesis, including cancer and diabetic retinopathy, in cooperation with many other signaling partners; thus, VEGFR2 and its downstream signaling appear to be critical targets for the suppression of these diseases. VEGF and VEGFR2-mediated survival signaling is critical to endothelial cell survival, maintenance of the vasculature and alveolar structure and regeneration of lung tissue. Reduced VEGF and VEGFR2 expression in emphysematous lungs has been linked to increased endothelial cell death and vascular regression.