|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human LILRB3 ORF mammalian expression plasmid, C-GFPSpark tag||HG11978-ACG|
|Human LILRB3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11978-ACR|
|Human LILRB3 ORF mammalian expression plasmid, C-Flag tag||HG11978-CF|
|Human LILRB3 ORF mammalian expression plasmid, C-His tag||HG11978-CH|
|Human LILRB3 ORF mammalian expression plasmid, C-Myc tag||HG11978-CM|
|Human LILRB3 ORF mammalian expression plasmid, C-HA tag||HG11978-CY|
|Human LILRB3 Gene cDNA clone plasmid||HG11978-M|
|Human LILRB3 ORF mammalian expression plasmid, N-Flag tag||HG11978-NF|
|Human LILRB3 ORF mammalian expression plasmid, N-His tag||HG11978-NH|
|Human LILRB3 ORF mammalian expression plasmid, N-Myc tag||HG11978-NM|
|Human LILRB3 ORF mammalian expression plasmid, N-HA tag||HG11978-NY|
|Human LILRB3 natural ORF mammalian expression plasmid||HG11978-UT|
|Learn more about expression Vectors|
Leukocyte immunoglobulin-like receptor subfamily B member 3, also known as Leukocyte immunoglobulin-like receptor 3, Immunoglobulin-like transcript 5, Monocyte inhibitory receptor HL9, CD85 antigen-like family member A, CD85a and LILRB3, is a single-pass type I membrane protein which belongs to the leukocyte receptor cluster (LRC) present on 19q13.4. LILRB3 / CD85a contains four Ig-like C2-type (immunoglobulin-like) domains. LILRB3 / CD85a contains three copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. LILRB3 / CD85a is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found.